-to long term carbon stockage (using atmospheric CO2, important in the
-context of climate change). Chloroplast found in Eucaryots have an endosymbiotic origin, meaning
-that they are a fusion of a photosynthetic bacteria (Cyanobacteria) and
-a eucaryotic cell (enable to produce organic matter = heterotrophic).
-This fusion or First Endiosymbiosis ended in a Great diversification of
-a lineage comprising Red Algae, Green Algae and Land Plants (terrestrial).
-Several Second Enbiosymbioses occurred then: two involving a Red
-Algae and other heterotrophic eucaryotes and giving birth to both Brown
-Algae and Dinoflagellates lineages; another involving a Green Algae and
-a heterotrophic eucaryot and giving birth to Euglens.
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+to long term carbon stockage (using atmospheric CO2) an important feature in the
+context of climate change. Chloroplasts found in Eucaryotes have an endosymbiotic origin, meaning
+that they from the incorporation of a photosynthetic bacteria (Cyanobacteria) within an eucaryotic cell. \\
+
+By the principle of phylogenetic classification, a mutation in the DNA shared by two to several taxa has a higher probability to be inherited from common ancestor than to have evolved independently. In such a process, shared changes in the genomes allow to build relationships between species. In the case of chloroplasts, an important category of changes in the genome is the loss of functional genes, when inoperant or when transferred to the nucleus. Thereby, we hypothesize that small number of gene losses among species indicates
+that these species are close to each other and belong to same lineage,
+while a large loss means that we have an evolutionary relationship
+between species from much more distant lineages. Phylogenetic relationships are mainly built by comparison of sets of coding and non-coding sequences. Phylogenies of photosynthetic plants are important to assess the origin of chloroplasts (REF) and the modalities of gene loss among lineages. These phylogenies are usually done using less than ten chloroplastic genes (REF), and some of them may not be conserved by evolution process for every taxa. As phylogenetic relationships inferred from data matrices complete for each species included and with the same evolution history are better assumptions, we selected core genomes for a new investigation of photosynthetic plants phylogeny. To depict the links between species clearly, we here intend to built a phylogenetic tree showing the relationships based on the distances among gene sequences of a core genome. The circumscription of the core chloroplast genomes for a given set of photosynthetic organisms needs bioinformatic tools for sequence annotation and comparison that we describe here.
+
+Other possible scientific questions to consider for introduction improvement:
+Which bioinformatic tools are necessary for genes comparison in selected complete chloroplast genomes? Which bioinformatic tools are necessary to build a phylogeny of numerous genes and species, etc?
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