2 DNA analysis techniques have received a lot of attention these last
3 years, because they play an important role in understanding genomes
4 evolution over time, and in phylogenetic and genetic analyses. Various
5 models of genomes evolution are based on the analysis of DNA
6 sequences, SNPs, mutations, and so on. We have recently investigated
7 the use of core (\emph{i.e.}, common genes) and pan genomes to infer
8 evolutionary information on a collection of 99~chloroplasts. In
9 particular, we have regarded methods to build a genes content
10 evolutionary tree using distances to core genome. However, the
11 production of reliable core and pan genomes is not an easy task, due
12 to error annotations. We will first compare different approaches to
13 construct such a tree using fully annoted genomes provided by NCBI and
14 Dogma, followed by a gene quality control among the common genes. Then
15 we will explain how, by comparing sequences from Dogma with NCBI
16 contents, we achieved to identify the genes that play a key role in
17 the dynamics of genomes evolution.
19 \textbf{Keywords:} genome evolution, phylogenetic tree, core genes, evolution tree, genome annotation