2 DNA analysis techniques have received a lot of attention these last
3 years, because they play an important role in understanding
4 genomes evolution over time, and in phylogenetic and genetic analyses. Various models
5 of genomes evolution are based on the analysis of DNA sequences, SNPs,
6 mutations, and so on. We have recently investigated the use of
7 core (\emph{i.e.}, common genes) and pan genomes to infer evolutionary information
8 on a collection of 107 chloroplasts. In particular,
9 we have regarded methods to build a genes content evolutionary tree using
10 distances to core genome. However,
11 the production of reliable core and pan genomes is not an easy task,
12 due to error annotations of the NCBI. The presentation will then
13 consist in various compared approaches to construct such a tree using
14 fully annotated genomes by NCBI and Dogma, followed by a gene quality
15 control among the common genes. We will finally explain how, by comparing
16 sequences from Dogma with NCBI contents, we achieved to identify the
17 genes that play a key role in the dynamics of genomes evolution. \\
19 \textbf{Keywords:} genome evolution, phylogenetic tree, core genes, evolution tree, genome annotation