2 title={Genomic analysis of smooth tubercle bacilli provides insights into ancestry and pathoadaptation of Mycobacterium tuberculosis},
3 url={http://www.nature.com/ng/journal/v45/n2/full/ng.2517.html},
7 journal={Nature Genetics},
8 author={Philip Supply and Michael Marceau and Sophie Mangenot and David Roche and Carine Rouanet and Varun Khanna and Laleh Majlessi and Alexis Criscuolo and Julien Tap and Alexandre Pawlik},
13 title={Yeast Ancestral Genome Reconstructions: The Possibilities of Computational Methods II},
14 author={Cedric Chauve and Haris Gavranovic and Aida Ouangraoua and Eric Tannier},
15 journal={Journal of Computational Biology},
21 DOI={10.1089/cmb.2010.0092}
24 @article{parra2007cegma,
25 title={CEGMA: a pipeline to accurately annotate core genes in eukaryotic genomes},
26 author={Parra, Genis and Bradnam, Keith and Korf, Ian},
27 journal={Bioinformatics},
32 publisher={Oxford Univ Press}
35 @article{parra2000geneid,
36 title={Geneid in drosophila},
37 author={Parra, Gen{\'\i}s and Blanco, Enrique and Guig{\'o}, Roderic},
38 journal={Genome research},
43 publisher={Cold Spring Harbor Lab}
46 @article{birney2004genewise,
47 title={GeneWise and genomewise},
48 author={Birney, Ewan and Clamp, Michele and Durbin, Richard},
49 journal={Genome research},
54 publisher={Cold Spring Harbor Lab}
57 @article{chapman2000biopython,
58 title={Biopython: Python tools for computational biology},
59 author={Chapman, Brad and Chang, Jeffrey},
60 journal={ACM SIGBIO Newsletter},
70 isbn={978-3-642-04743-5},
71 booktitle={Comparative Genomics},
73 series={Lecture Notes in Computer Science},
74 editor={Ciccarelli, FrancescaD. and Miklós, István},
75 doi={10.1007/978-3-642-04744-2_1},
76 title={Yeast Ancestral Genome Reconstructions: The Possibilities of Computational Methods},
77 url={http://dx.doi.org/10.1007/978-3-642-04744-2_1},
78 publisher={Springer Berlin Heidelberg},
79 author={Tannier, Eric},
83 @article{gansner2002drawing,
84 title={Drawing graphs with dot},
85 author={Gansner, Emden and Koutsofios, Eleftherios and North, Stephen},
86 journal={Retrieved June},
92 @article{10.1371/journal.pone.0052841,
93 author = {Blouin, Yann AND Hauck, Yolande AND Soler, Charles AND Fabre, Michel AND Vong, Rithy AND Dehan, Céline AND Cazajous, Géraldine AND Massoure, Pierre-Laurent AND Kraemer, Philippe AND Jenkins, Akinbowale AND Garnotel, Eric AND Pourcel, Christine AND Vergnaud, Gilles},
95 publisher = {Public Library of Science},
96 title = {Significance of the Identification in the Horn of Africa of an Exceptionally Deep Branching <italic>Mycobacterium tuberculosis</italic> Clade},
100 url = {http://dx.doi.org/10.1371%2Fjournal.pone.0052841},
102 abstract = {<p>Molecular and phylogeographic studies have led to the definition within the <italic>Mycobacterium tuberculosis</italic> complex (MTBC) of a number of geotypes and ecotypes showing a preferential geographic location or host preference. The MTBC is thought to have emerged in Africa, most likely the Horn of Africa, and to have spread worldwide with human migrations. Under this assumption, there is a possibility that unknown deep branching lineages are present in this region. We genotyped by spoligotyping and multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) 435 MTBC isolates recovered from patients. Four hundred and eleven isolates were collected in the Republic of Djibouti over a 12 year period, with the other 24 isolates originating from neighbouring countries. All major <italic>M. tuberculosis</italic> lineages were identified, with only two <italic>M. africanum</italic> and one <italic>M. bovis</italic> isolates. Upon comparison with typing data of worldwide origin we observed that several isolates showed clustering characteristics compatible with new deep branching. Whole genome sequencing (WGS) of seven isolates and comparison with available WGS data from 38 genomes distributed in the different lineages confirms the identification of ancestral nodes for several clades and most importantly of one new lineage, here referred to as lineage 7. Investigation of specific deletions confirms the novelty of this lineage, and analysis of its precise phylogenetic position indicates that the other three superlineages constituting the MTBC emerged independently but within a relatively short timeframe from the Horn of Africa. The availability of such strains compared to the predominant lineages and sharing very ancient ancestry will open new avenues for identifying some of the genetic factors responsible for the success of the modern lineages. Additional deep branching lineages may be readily and efficiently identified by large-scale MLVA screening of isolates from sub-Saharan African countries followed by WGS analysis of a few selected isolates.</p>},
104 doi = {10.1371/journal.pone.0052841}
107 @article{zafar2002coregenes,
108 title={CoreGenes: A computational tool for identifying and cataloging},
109 author={Zafar, Nikhat and Mazumder, Raja and Seto, Donald},
110 journal={BMC bioinformatics},
115 publisher={BioMed Central Ltd}
119 AUTHOR = {Gomez-Valero, Laura and Rocha, Eduardo P C and Latorre, Amparo and Silva, Francisco J},
120 TITLE = {Reconstructing the ancestor of Mycobacterium leprae: the dynamics of gene loss and genome reduction.},
121 JOURNAL = {Genome Res},
126 URL = {http://www.biomedsearch.com/nih/Reconstructing-ancestor-Mycobacterium-leprae-dynamics/17623808.html},
127 PubMedID = {17623808},
131 author = {Eisen, Jonathan A},
132 journal = {PLoS Biol},
133 publisher = {Public Library of Science},
134 title = {Environmental Shotgun Sequencing: Its Potential and Challenges for Studying the Hidden World of Microbes},
138 url = {http://dx.doi.org/10.1371%2Fjournal.pbio.0050082},
141 <p>Environmental shotgun sequencing promises to reveal novel and fundamental insights into the hidden world of microbes, but the complexity of analysis required to realize this potential poses unique interdisciplinary challenges.</p>
144 doi = {10.1371/journal.pbio.0050082}
147 AUTHOR = {Stacia K. Wyman, Robert K. Jansen and Jeffrey L. Boore},
148 TITLE = {Automatic annotation of organellar genomes
150 JOURNAL = {BIOINFORMATICS, oxford Press},
155 URL={http://www.biosci.utexas.edu/ib/faculty/jansen/pubs/Wyman%20et%20al.%202004.pdf},
157 @article{guindon2005phyml,
158 title={PHYML Online—a web server for fast maximum likelihood-based phylogenetic inference},
159 author={Guindon, Stephane and Lethiec, Franck and Duroux, Patrice and Gascuel, Olivier},
160 journal={Nucleic acids research},
165 publisher={Oxford Univ Press}
168 @article{goloboff2008tnt,
169 title={TNT, a free program for phylogenetic analysis},
170 author={Goloboff, Pablo A and Farris, James S and Nixon, Kevin C},
171 journal={Cladistics},
176 publisher={Wiley Online Library}
179 @article{stamatakis2008raxml,
180 title={The RAxML 7.0. 4 Manual},
181 author={Stamatakis, Alexandros},
182 journal={Department of Computer Science. Ludwig-Maximilians-Universit{\"a}t M{\"u}nchen},
185 @article{stamatakis2005raxml,
186 title={RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees},
187 author={Stamatakis, Alexandros and Ludwig, Thomas and Meier, Harald},
188 journal={Bioinformatics},
193 publisher={Oxford Univ Press}
198 author = {Bakke, Peter AND Carney, Nick AND DeLoache, Will AND Gearing, Mary AND Ingvorsen, Kjeld AND Lotz, Matt AND McNair, Jay AND Penumetcha, Pallavi AND Simpson, Samantha AND Voss, Laura AND Win, Max AND Heyer, Laurie J. AND Campbell, A. Malcolm},
199 journal = {PLoS ONE},
200 publisher = {Public Library of Science},
201 title = {Evaluation of Three Automated Genome Annotations for <italic>Halorhabdus utahensis</italic>},
205 url = {http://dx.doi.org/10.1371%2Fjournal.pone.0006291},
208 <p>Genome annotations are accumulating rapidly and depend heavily on automated annotation systems. Many genome centers offer annotation systems but no one has compared their output in a systematic way to determine accuracy and inherent errors. Errors in the annotations are routinely deposited in databases such as NCBI and used to validate subsequent annotation errors. We submitted the genome sequence of halophilic archaeon <italic>Halorhabdus utahensis</italic> to be analyzed by three genome annotation services. We have examined the output from each service in a variety of ways in order to compare the methodology and effectiveness of the annotations, as well as to explore the genes, pathways, and physiology of the previously unannotated genome. The annotation services differ considerably in gene calls, features, and ease of use. We had to manually identify the origin of replication and the species-specific consensus ribosome-binding site. Additionally, we conducted laboratory experiments to test <italic>H. utahensis</italic> growth and enzyme activity. Current annotation practices need to improve in order to more accurately reflect a genome's biological potential. We make specific recommendations that could improve the quality of microbial annotation projects.</p>
211 doi = {10.1371/journal.pone.0006291}
214 @article{altschul1990basic,
215 title={Basic local alignment search tool},
216 author={Altschul, Stephen F and Gish, Warren and Miller, Webb and Myers, Eugene W and Lipman, David J},
217 journal={Journal of molecular biology},
226 title={DNASTAR- GenVision Software for Genomic Visualizations},
228 url = {http://www.dnastar.com/products/genvision.php}