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33 %% \author{\IEEEauthorblockN{Authors Name/s per 1st Affiliation (Author)}
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48 \title{Using FPGAs for high speed and real time cantilever deflection estimation}
49 \author{\IEEEauthorblockN{Raphaël Couturier\IEEEauthorrefmark{1}, Stéphane Domas\IEEEauthorrefmark{1}, Gwenhaël Goavec-Merou\IEEEauthorrefmark{2} and Michel Lenczner\IEEEauthorrefmark{2}}
50 \IEEEauthorblockA{\IEEEauthorrefmark{1}FEMTO-ST, DISC, University of Franche-Comte, Belfort, France\\
51 \{raphael.couturier,stephane.domas\}@univ-fcomte.fr}
52 \IEEEauthorblockA{\IEEEauthorrefmark{2}FEMTO-ST, Time-Frequency, University of Franche-Comte, Besançon, France\\
53 \{michel.lenczner@utbm.fr,gwenhael.goavec@trabucayre.com}
69 {\it keywords}: FPGA, cantilever, interferometry.
72 \section{Introduction}
74 Cantilevers are used inside atomic force microscope which provides high
75 resolution images of surfaces. Several technics have been used to measure the
76 displacement of cantilevers in litterature. For example, it is possible to
77 determine accurately the deflection with different mechanisms.
78 In~\cite{CantiPiezzo01}, authors used piezoresistor integrated into the
79 cantilever. Nevertheless this approach suffers from the complexity of the
80 microfabrication process needed to implement the sensor in the cantilever.
81 In~\cite{CantiCapacitive03}, authors have presented an cantilever mechanism
82 based on capacitive sensing. This kind of technic also involves to instrument
83 the cantiliver which result in a complex fabrication process.
85 In this paper our attention is focused on a method based on interferometry to
86 measure cantilevers' displacements. In this method cantilevers are illuminated
87 by an optic source. The interferometry produces fringes on each cantilevers
88 which enables to compute the cantilever displacement. In order to analyze the
89 fringes a high speed camera is used. Images need to be processed quickly and
90 then a estimation method is required to determine the displacement of each
91 cantilever. In~\cite{AFMCSEM11}, the authors have used an algorithm based on
92 spline to estimate the cantilevers' positions.
93 %%RAPH : ce qui est génant c'est qu'ils ne parlent pas de spline dans ce papier...
94 The overall process gives
95 accurate results but all the computation are performed on a standard computer
96 using labview. Consequently, the main drawback of this implementation is that
97 the computer is a bootleneck in the overall process. In this paper we propose to
98 use a method based on least square and to implement all the computation on a
101 The remainder of the paper is organized as follows. Section~\ref{sec:measure}
102 describes more precisely the measurement process. Our solution based on the
103 least square method and the implementation on FPGA is presented in
104 Section~\ref{sec:solus}. Experimentations are described in
105 Section~\ref{sec:results}. Finally a conclusion and some perspectives are
110 %% quelques ref commentées sur les calculs basés sur l'interférométrie
112 \section{Measurement principles}
115 In order to develop simple, cost effective and user-friendly probe arrays,
116 authors of ~\cite{AFMCSEM11} have developped a system based of interferometry.
119 \subsection{Architecture}
121 %% description de l'architecture générale de l'acquisition d'images
122 %% avec au milieu une unité de traitement dont on ne précise pas ce
125 %% image tirée des expériences.
127 \subsection{Cantilever deflection estimation}
130 As shown on image \ref{img:img-xp}, each cantilever is covered by
131 interferometric fringes. The fringes will distort when cantilevers are
132 deflected. Estimating the deflection is done by computing this
133 distortion. For that, (ref A. Meister + M Favre) proposed a method
134 based on computing the phase of the fringes, at the base of each
135 cantilever, near the tip, and on the base of the array. They assume
136 that a linear relation binds these phases, which can be use to
137 "unwrap" the phase at the tip and to determine the deflection.\\
139 More precisely, segment of pixels are extracted from images taken by a
140 high-speed camera. These segments are large enough to cover several
141 interferometric fringes and are placed at the base and near the tip of
142 the cantilevers. They are called base profile and tip profile in the
143 following. Furthermore, a reference profile is taken on the base of
144 the cantilever array.
146 The pixels intensity $I$ (in gray level) of each profile is modelized by :
150 I(x) = ax+b+A.cos(2\pi f.x + \theta)
153 where $x$ is the position of a pixel in its associated segment.
155 The global method consists in two main sequences. The first one aims
156 to determin the frequency $f$ of each profile with an algorithm based
157 on spline interpolation (see section \ref{algo-spline}). It also
158 computes the coefficient used for unwrapping the phase. The second one
159 is the acquisition loop, while which images are taken at regular time
160 steps. For each image, the phase $\theta$ of all profiles is computed
161 to obtain, after unwrapping, the deflection of cantilevers.
163 \subsection{Design goals}
166 If we put aside some hardware issues like the speed of the link
167 between the camera and the computation unit, the time to deserialize
168 pixels and to store them in memory, ... the phase computation is
169 obviously the bottle-neck of the whole process. For example, if we
170 consider the camera actually in use, an exposition time of 2.5ms for
171 $1024\times 1204$ pixels seems the minimum that can be reached. For a
172 $10\times 10$ cantilever array, if we neglect the time to extract
173 pixels, it implies that computing the deflection of a single
174 cantilever should take less than 25$\mu$s, thus 12.5$\mu$s by phase.\\
176 In fact, this timing is a very hard constraint. Let consider a very
177 small programm that initializes twenty million of doubles in memory
178 and then does 1000000 cumulated sums on 20 contiguous values
179 (experimental profiles have about this size). On an intel Core 2 Duo
180 E6650 at 2.33GHz, this program reaches an average of 155Mflops. It
181 implies that the phase computation algorithm should not take more than
182 $240\times 12.5 = 1937$ floating operations. For integers, it gives
185 %% to be continued ...
187 %% � faire : timing de l'algo spline en C avec atan et tout le bordel.
192 \section{Proposed solution}
196 \subsection{FPGA constraints}
198 %% contraintes imposées par le FPGA : algo pipeline/parallele, pas d'op math complexe, ...
201 \subsection{Considered algorithms}
203 Two solutions have been studied to achieve phase computation. The
204 original one, proposed by A. Meister and M. Favre, is based on
205 interpolation by splines. It allows to compute frequency and
206 phase. The second one, detailed in this article, is based on a
207 classical least square method but suppose that frequency is already
210 \subsubsection{Spline algorithm}
211 \label{sec:algo-spline}
212 Let consider a profile $P$, that is a segment of $M$ pixels with an
213 intensity in gray levels. Let call $I(x)$ the intensity of profile in $x
216 At first, only $M$ values of $I$ are known, for $x = 0, 1,
217 \ldots,M-1$. A normalisation allows to scale known intensities into
218 $[-1,1]$. We compute splines that fit at best these normalised
219 intensities. Splines are used to interpolate $N = k\times M$ points
220 (typically $k=3$ is sufficient), within $[0,M[$. Let call $x^s$ the
221 coordinates of these $N$ points and $I^s$ their intensities.
223 In order to have the frequency, the mean line $a.x+b$ (see equation \ref{equ:profile}) of $I^s$ is
224 computed. Finding intersections of $I^s$ and this line allow to obtain
225 the period thus the frequency.
227 The phase is computed via the equation :
229 \theta = atan \left[ \frac{\sum_{i=0}^{N-1} sin(2\pi f x^s_i) \times I^s(x^s_i)}{\sum_{i=0}^{N-1} cos(2\pi f x^s_i) \times I^s(x^s_i)} \right]
232 Two things can be noticed. Firstly, the frequency could also be
233 obtained using the derivates of spline equations, which only implies
234 to solve quadratic equations. Secondly, frequency of each profile is
235 computed a single time, before the acquisition loop. Thus, $sin(2\pi f
236 x^s_i)$ and $cos(2\pi f x^s_i)$ could also be computed before the loop, which leads to a
237 much faster computation of $\theta$.
239 \subsubsection{Least square algorithm}
241 Assuming that we compute the phase during the acquisition loop,
242 equation \ref{equ:profile} has only 4 parameters :$a, b, A$, and
243 $\theta$, $f$ and $x$ being already known. Since $I$ is non-linear, a
244 least square method based an Gauss-newton algorithm must be used to
245 determine these four parameters. Since it is an iterative process
246 ending with a convergence criterion, it is obvious that it is not
247 particularly adapted to our design goals.
249 Fortunatly, it is quite simple to reduce the number of parameters to
250 only $\theta$. Let $x^p$ be the coordinates of pixels in a segment of
251 size $M$. Thus, $x^p = 0, 1, \ldots, M-1$. Let $I(x^p)$ be their
252 intensity. Firstly, we "remove" the slope by computing :
254 \[I^{corr}(x^p) = I(x^p) - a.x^p - b\]
256 Since linear equation coefficients are searched, a classical least
257 square method can be used to determine $a$ and $b$ :
259 \[a = \frac{covar(x^p,I(x^p))}{var(x^p)} \]
261 Assuming an overlined symbol means an average, then :
263 \[b = \overline{I(x^p)} - a.\overline{{x^p}}\]
265 Let $A$ be the amplitude of $I^{corr}$, i.e.
267 \[A = \frac{max(I^{corr}) - min(I^{corr})}{2}\]
269 Then, the least square method to find $\theta$ is reduced to search the minimum of :
271 \[\sum_{i=0}^{M-1} \left[ cos(2\pi f.i + \theta) - \frac{I^{corr}(i)}{A} \right]^2\]
273 It is equivalent to derivate this expression and to solve the following equation :
276 2\left[ cos\theta \sum_{i=0}^{M-1} I^{corr}(i).sin(2\pi f.i) + sin\theta \sum_{i=0}^{M-1} I^{corr}(i).cos(2\pi f.i)\right] \\
277 - A\left[ cos2\theta \sum_{i=0}^{M-1} sin(4\pi f.i) + sin2\theta \sum_{i=0}^{M-1} cos(4\pi f.i)\right] = 0
280 Several points can be noticed :
282 \item As in the spline method, some parts of this equation can be
283 computed before the acquisition loop. It is the case of sums that do
284 not depend on $\theta$ :
286 \[ \sum_{i=0}^{M-1} sin(4\pi f.i), \sum_{i=0}^{M-1} cos(4\pi f.i) \]
288 \item Lookup tables for $sin(2\pi f.i)$ and $cos(2\pi f.i)$ can also be
291 \item The simplest method to find the good $\theta$ is to discretize
292 $[-\pi,\pi]$ in $N$ steps, and to search which step leads to the
293 result closest to zero. By the way, three other lookup tables can
294 also be computed before the loop :
296 \[ sin \theta, cos \theta, \left[ cos 2\theta \sum_{i=0}^{M-1} sin(4\pi f.i) + sin 2\theta \sum_{i=0}^{M-1} cos(4\pi f.i)\right] \]
298 \item This search can be very fast using a dichotomous process in $log_2(N)$
302 Finally, the whole summarizes in an algorithm (called LSQ in the following) in two parts, one before and one during the acquisition loop :
304 \caption{LSQ algorithm - before acquisition loop.}
305 \label{alg:lsq-before}
307 $M \leftarrow $ number of pixels of the profile\\
308 I[] $\leftarrow $ intensities of pixels\\
309 $f \leftarrow $ frequency of the profile\\
310 $s4i \leftarrow \sum_{i=0}^{M-1} sin(4\pi f.i)$\\
311 $c4i \leftarrow \sum_{i=0}^{M-1} cos(4\pi f.i)$\\
312 $nb_s \leftarrow $ number of discretization steps of $[-\pi,\pi]$\\
314 \For{$i=0$ to $nb_s $}{
315 $\theta \leftarrow -\pi + 2\pi\times \frac{i}{nb_s}$\\
316 lut\_sin[$i$] $\leftarrow sin \theta$\\
317 lut\_cos[$i$] $\leftarrow cos \theta$\\
318 lut\_A[$i$] $\leftarrow cos 2 \theta \times s4i + sin 2 \theta \times c4i$\\
319 lut\_sinfi[$i$] $\leftarrow sin (2\pi f.i)$\\
320 lut\_cosfi[$i$] $\leftarrow cos (2\pi f.i)$\\
325 \caption{LSQ algorithm - during acquisition loop.}
326 \label{alg:lsq-during}
328 $\bar{x} \leftarrow \frac{M-1}{2}$\\
329 $\bar{y} \leftarrow 0$, $x_{var} \leftarrow 0$, $xy_{covar} \leftarrow 0$\\
330 \For{$i=0$ to $M-1$}{
331 $\bar{y} \leftarrow \bar{y} + $ I[$i$]\\
332 $x_{var} \leftarrow x_{var} + (i-\bar{x})^2$\\
334 $\bar{y} \leftarrow \frac{\bar{y}}{M}$\\
335 \For{$i=0$ to $M-1$}{
336 $xy_{covar} \leftarrow xy_{covar} + (i-\bar{x}) \times (I[i]-\bar{y})$\\
338 $slope \leftarrow \frac{xy_{covar}}{x_{var}}$\\
339 $start \leftarrow y_{moy} - slope\times \bar{x}$\\
340 \For{$i=0$ to $M-1$}{
341 $I[i] \leftarrow I[i] - start - slope\times i$\tcc*[f]{slope removal}\\
344 $I_{max} \leftarrow max_i(I[i])$, $I_{min} \leftarrow min_i(I[i])$\\
345 $amp \leftarrow \frac{I_{max}-I_{min}}{2}$\\
347 $Is \leftarrow 0$, $Ic \leftarrow 0$\\
348 \For{$i=0$ to $M-1$}{
349 $Is \leftarrow Is + I[i]\times $ lut\_sinfi[$i$]\\
350 $Ic \leftarrow Ic + I[i]\times $ lut\_cosfi[$i$]\\
353 $\theta \leftarrow -\pi$\\
354 $val_1 \leftarrow 2\times \left[ Is.\cos(\theta) + Ic.\sin(\theta) \right] - amp\times \left[ c4i.\sin(2\theta) + s4i.\cos(2\theta) \right]$\\
355 \For{$i=1-n_s$ to $n_s$}{
356 $\theta \leftarrow \frac{i.\pi}{n_s}$\\
357 $val_2 \leftarrow 2\times \left[ Is.\cos(\theta) + Ic.\sin(\theta) \right] - amp\times \left[ c4i.\sin(2\theta) + s4i.\cos(2\theta) \right]$\\
359 \lIf{$val_1 < 0$ et $val_2 >= 0$}{
360 $\theta_s \leftarrow \theta - \left[ \frac{val_2}{val_2-val_1}\times \frac{\pi}{n_s} \right]$\\
362 $val_1 \leftarrow val_2$\\
368 \subsubsection{Comparison}
370 \subsection{VHDL design paradigms}
372 \subsection{VHDL implementation}
374 \section{Experimental results}
380 \section{Conclusion and perspectives}
383 \bibliographystyle{plain}
384 \bibliography{biblio}